EDUCATION

EXPERIENCE 

HONORS AND MEMBERSHIPS  

SELECTED PUBLICATIONS  

Nolan, J.P., R.G. Posner, R. Habbersett, J.C. Martin, L.A. Sklar (1995) A rapid mix flow cytometer with subsecond kinetic resolution. Cytometry 21:223-229.

Nolan, J.P., L.A. Sklar (1998) The emergence of flow cytometry for sensitive, real-time measurements of molecular interactions.  Nature Biotechnology 16:633-638.

Nolan, J.P., S. Lauer, E.R. Prossnitz, L.A. Sklar (1999) Flow cytometry: a versatile tool for all stages of drug discovery. Drug Discovery Today 4:173-180.

Graves, S.W., R.C. Habbersett, J.P. Nolan (2001) A dynamic inline sample thermoregulation unit for flow cytometry. Cytometry 43:23-30.

Nolan, J.P., L.A. Sklar (2002) Suspension array technology: Evolution of the flat array paradigm. Trends in Biotechnology 20:9-12.

Lauer, S., B. Goldstein, R. Nolan, J.P. Nolan (2002) Analysis of cholera toxin-ganglioside interactions by flow cytometry. Biochemistry 41:1742-1751.

Cleland C.A., P.S. White, A. Deshpande, M. Wolinsky, J. Song, J.P. Nolan (2004) Rational design of nucleic acid signatures for the detection, identification, and characterization of microbial pathogens. Expert Reviews in Molecular Diagnostics 4: 89-101.

COMPLETE PUBLICATIONS

1. Hammerstedt, R.H., J.K. Graham J.P. Nolan (1990) Cryopreservation of mammalian sperm: what we ask them to survive. Journal of Andrology 11: 73-88.  [Full Text]

2. Graham, J.K., J.P. Nolan, R.H. Hammerstedt (1991) Effect of dilauroyl-phosphatidylcholine liposomes on motility, induction of the acrosome reaction, and subsequent egg penetration by epididymal ram sperm. Biology of Reproduction 44: 1092-1099.  [Full Text]

3. Nolan, J.P., J.K. Graham, R.H. Hammerstedt (1992) Artificial induction of exocytosis in bull sperm. Archives of Biochemistry and Biophysics 292: 311-322.

4. Andrews, J.C., J.P. Nolan, R.H. Hammerstedt, B.D. Bavister (1994) Role of zinc during hamster sperm capacitation. Biology of Reproduction 51: 1238-1247.  [Full Text]

5. Nolan, J.P., S.F. Magargee, R.G. Posner, R.H. Hammerstedt (1995) Flow cytometric analysis of transmembrane phospholipid movement in bull sperm. Biochemistry 34: 3907-3915.  [Full Text]

6. Andrews, J.C., J.P. Nolan, R.H. Hammerstedt, B.D. Bavister (1995) Characterization of N-(6-methoxy-8-quinolyl)-p-toluenesulfonamide for the detection of zinc in hamster sperm. Cytometry 21:153-159.

7. Nolan, J.P., R.G. Posner, R. Habbersett, J.C. Martin, L.A. Sklar (1995) A rapid mix flow cytometer with subsecond kinetic resolution. Cytometry 21:223-229.  [Full Text]

8. Shen, B., J.P. Nolan, L.A. Sklar, M.S. Park (1996) Essential amino acids for substrate binding and catalysis of human flap endonuclease-1. Journal of Biological Chemistry 271:9173-9176.  [Full Text]

9. Nolan, J.P., Shen, B., M.S. Park, L.A. Sklar (1996) Kinetic analysis of human flap endonuclease-1 by flow cytometry. Biochemistry 35:11668-11676.  [Full Text]

10. Robbins D.L., J.P. Nolan., J.H. Jett, L.A. Sklar, R.A. Keller (1997) Analysis of individual lipoproteins and liposomes.  Proceedings SPIE 2985:213-222.  [Full Text]

11. Roslaniec, M.C., C.S. Bell-Prince, H.A. Crissman, J.J. Fawcett, P.M. Goodwin, R. Habbersett, J.H. Jett, R.A. Keller, J.C. Martin, B.L. Marrone, J.P. Nolan, M.S. Park, B.L. Sailer, L.A.Sklar, J.A. Steinkamp, L.S. Cram (1997) New flow cytometric technologies for the 21st century. Human Cell 10:3-10.

12. Nolan, J.P., R.H. Hammerstedt (1997) Regulation of membrane stability and the acrosome reaction in mammalian sperm. FASEB Journal 11:670-682.  [Full Text]

13. Shen B., J.P. Nolan, L.A. Sklar, M.S. Park (1997) Functional analysis of point mutations in human flap endonuclease-1 active site. Nucleic Acids Research 25:3332-3338.  [Full Text]

14. Nolan, J.P. H. Cai, K. Kommander, B. Lehnert, R. Nolan, M. Park, T. Ruscetti, B. Shen, L.A. Sklar (1998) Real-Time Analysis of Molecular Assembly by Kinetic Flow Cytometry.  In Advances in Optical Biophysics. (Eds. J.R. Lakowicz and J.B.A. Ross) Proceedings SPIE 3256:114-121.  [Full Text]

15. Cai H., K. Kommander, P.S. White, R. Keller, J.P. Nolan (1998) Flow Cytometry-Based Hybridization and Polymorphism Detection and Analysis. In, Advances in Optical Biophysics (Eds. J.R. Lakowicz and J.B.A. Ross) Proceedings SPIE 3256:171-177.  [Full Text]

16. Nolan, J.P., L.A. Sklar (1998) The emergence of flow cytometry for sensitive, real-time measurements of molecular interactions.  Nature Biotechnology 16:633-638.  [Full Text]

17. Sklar L.A., L.C. Seamer, F. Kuckuck, R.G. Posner, E. Prossnitz, B. Edwards, J.P. Nolan (1998) Sample handling for molecular assembly in flow cytometry. In, Advances in Optical Biophysics (Eds. J.R. Lakowicz and J.B.A. Ross) Proceedings SPIE 3256:144-153.

18. Gudi S.R.P., J.P. Nolan, J.A. Frangos (1998) Modulation of GTPase activity of G proteins by fluid shear stress and phospholipid composition. Proceedings of the National Academy of Sciences 95: 2515-2519. [Full Text]

19. Song, X., J.P. Nolan, B Swanson (1998) Optical signal transduction triggered by protein-ligand binding: Detection of toxins using multivalent receptors. Journal of the American Chemical Society 120:4873-4874.  [Full Text]

20. Song, X., J.P. Nolan, B. Swanson (1998) An optical biosensor based on fluorescence self-quenching and energy transfer: Ultrasensitive and specific detection of protein toxins.  Journal of the American Chemical Society 120:11514-11515.  [Full Text]

21. Nolan, J.P., J.D. Chambers, L.A. Sklar (1998) Cytometric approaches to the study of receptors. In, Cytometric Cellular Analysis, Vol. 1. Phagocyte Function: A Guide for Research and Clinical Evaluation (Eds. J.P. Robinson and G. Babcock), pp 19-46. John Wiley and Sons, New York.

22. Frank, G, J. Qui, M. Somsouk, Y. Weng, L. Somsouk, J.P. Nolan, B. Shen (1998) Partial functional deficiency of E160D flap endonuclease-1 mutant in vitro and in vivo is due to defective cleavage of DNA substrates. Journal of Biological Chemistry 273:33064-33072.  [Full Text]

23. Buranda, T., G. Jones, J.P. Nolan, J. Keij, G.P. Lopez, L.A. Sklar (1999) Ligand-receptor dynamics at streptavidin coated particle surfaces: a flow cytometric and spectrofluorometric study.  Journal of Physical Chemistry B 103:3399-3410. 

24. Ambrose, W.P., P.M. Goodwin, J.P. Nolan (1999) Single molecule detection with total internal reflection excitation: comparing signal to background and total signal in different geometries.  Cytometry 36:224-231. [Full Text]

25. Gary, R., M.S. Park, J.P. Nolan, H.L. Cornelius, O.G. Korzyreva, H.P. Tran, K.S. Lobachev, M.A. Resnick, D.A. Gordenin (1999) A novel role in DNA metabolism for binding of Fen1/Rad27 to PCNA-and implications for genetic risk. Molecular and Cell Biology 19:5373-5382.  [Full Text]

26. Nolan, J.P., S. Lauer, E.R. Prossnitz, L.A. Sklar (1999) Flow cytometry: a versatile tool for all stages of drug discovery. Drug Discovery Today 4:173-180.  [Full Text]

27. Cai, H., P.S. White, D.C. Torney, A. Deshpande, Z. Wang, R.A. Keller, B. Marrone, J.P. Nolan (2000) Flow cytometry based minisequencing: a new platform for high throughput single nucleotide polymorphism analysis. Genomics 66:135-143.  [Full Text]

28. Yan, X, R.C. Habbersett, J.M. Cordek, J.P. Nolan, T.M. Yoshida, J.H. Jett, B.L. Marrone (2000) Development of a mechanism-based, DNA staining protocol using SYTOX Orange nucleic acid stain and DNA fragment sizing flow cytometry. Analytical Biochemistry 286, 138–148.g   [Full Text]

29. Graves, S.W., R.C. Habbersett, J.P. Nolan (2001) A dynamic inline sample thermoregulation unit for flow cytometry. Cytometry 43:23-30.  [Full Text]

30. Nolan J.P., Mandy F. (2001) Suspension array technology: New tools for gene and protein analysis. Cellular and Molecular Biology 47:1241-1256.

31. Song, X., J. Shi, J.P. Nolan, B. Swanson (2001) Detection of multivalent interactions through two-tiered energy transfer.  Analytical Biochemistry 291:133-141.  [Full Text]

32. Jett, J.H,  H. Cai, R.C. Habbersett, R.A. Keller, E.J. Larson, B.L. Marrone, J.P. Nolan, X. Song, B. Swanson, P.S. White (2001) Flow cytometry analysis techniques for high-throughput biodefense research.  In: Firepower in the Lab: Automation in the Fight Against Infectious Diseases and Bioterrorism.  (Layne SP, Beugelsdijk TJ, Patel CKN, eds).  Washington, DC: Joseph Henry Press, 193 - 201.

33. Nolan, J.P., P.S. White, H. Cai (2002) SNP scoring for drug discovery applications.  In, Integrated Drug Discovery Technologies (H.-Y. Mei and A. Czarnik, eds), pp 149-166, Marcel Dekker, New York.

34. Graves, S.W., J.P. Nolan, J.H. Jett, J.C. Martin, L.A. Sklar (2002) Effects of nozzle design on rapid delivery in flow cytometry. Cytometry 47:127-137.  [Full Text]

35. Nolan, J.P., L.A. Sklar (2002) Suspension array technology: Evolution of the flat array paradigm. Trends in Biotechnology 20:9-12.  [Full Text]

36. Lauer, S., B. Goldstein, R. Nolan, J.P. Nolan (2002) Analysis of cholera toxin-ganglioside interactions by flow cytometry. Biochemistry 41:1742-1751.  [Full Text]

37. Sklar, L.A., B. Edwards, S.W. Graves, J.P. Nolan, E. Prossnitz (2002) Flow cytometric analysis of ligand-receptor interactions and molecular assemblies. Annual Review of Biophysics and Biomolecular Structure 31:97-119.  [Full Text]

38. Graves, S.W., R.C. Habbersett, J.P. Nolan (2002) Dynamic temperature control of the sample in flow cytometry. Current Protocols in Cytometry 1.18 (J.P. Robinson, editor), John Wiley and Sons.

39. Lauer, S., J.P. Nolan (2002) Development and characterization of Ni-NTA microspheres. Cytometry 48:136-145.  [Full Text]

40. Green, L.D. , H.Cai, D.C. Torney, D.J. Wood, F.J. Uribe-Romeo, L. Kaderali, J.P. Nolan, P.S. White (2002) High-throughput SNP Scoring with GAMMArrays:  Genomic Analysis using Multiplexed Microsphere Arrays.  In Biomedical Nanotechnology Architectures and Applications (Eds. D. J. Bornhop; D.d A. Dunn; R.P. Mariella; C. J. Murphy; D. V. Nicolau; S. Nie; M. Palmer; R. Raghavachari) Proc. SPIE Vol. 4626, p. 308-315.

41. Nolan J.P., White PS (2002) Biosensor platforms and assays.  Proceedings of BTR 2002: Unified Science & Technology for Reducing Biological Threats & Countering Terrorism.

42. Kaderali, L., A. Deshpande, J.P. Nolan, P.S. White (2003) Primer design for multiplexed genotyping. Nucleic Acids Research 31: 1796-1802.  [Full Text]

43. Kraus, R.H., M.A. Espy, A.N. Matlachov, T.J. Matsson, C. Carr, J.C. Martin, J.P. Nolan, S. Graves, A.C. Bergstrom, M.D. Ward, P. Grodzinski (2003) Bioassay with magnetic particles in flow: a method for highly parallel molecular separations of complex biological systems. EUCAS 2003 Proceedings.

44. Nolan, R.L., H. Cai, J.P., Nolan, J.P., PM Goodwin (2003) A simple quenching method for fluorescence background reduction and its application to the direct, quantitative detection of specific mRNA. Analytical Chemistry 75: 6236-6243.  [Full Text]

45. Cleland C.A., P.S. White, A. Deshpande, M. Wolinsky, J. Song, J.P. Nolan (2004) Rational design of nucleic acid signatures for the detection, identification, and characterization of microbial pathogens. Expert Reviews in Molecular Diagnostics 4: 89-101.  [Full Text]

46. Yan, X., E.G. Schielke, K.M. Grace, C. Hassell, B.L. Marrone, J.P. Nolan (2004) Microsphere-based duplexed immunoassay for influenza virus typing by flow cytometry. J. Immunological Methods 284:27-38.  [Full Text]

47. Gerena, Y., J.P. Nolan, L. Wang, A. Schwartz, E. Fernandez-Repollet (2004) Quantification of EGFP expression on Molt-4 cells using calibration standards. Cytometry Part A:60A21-28.  [Full Text]

48. Deshpande, A., J.P. Nolan†, P.S. White, Y.E. Valdez, C.L. Peyton, W.C. Hunt, C.M. Wheeler (2005) TNF alpha promoter polymorphisms and susceptibility to HPV16-associated cervical cancer. Journal of Infectious Disease 191:969-976. †Corresponding author. [Full Text]

49. Graves S.W., T.A. Woods, J.P. Nolan (2005) Direct Flourescent Staining and Analysis of Proteins on Microspheres Using CBQCA. Cytometry 65A: 50-58.  [Full Text]

50. Yan, X., R.C. Habbersett, J.P. Nolan, T.M. Yoshida, J.H. Jett, B.L. Marrone (2005) Probing the kinetics of SYTOX Orange and PicoGreen stains binding to double-stranded DNA with implications for DNA analysis. Analytical Chemistry 77:3554-3562.  [Full Text]

51. Gramaglia I., H. Sahlin, J.P. Nolan, J. A. Frangos, M. Intaglietta, H. C. van der Heyde (2005) Cell- rather than antibody-mediated immunity leads to the development of profound thrombocytopenia during experimental Plasmodium berghei malaria. Journal of Immunology 175: 7699-7707.  [Full Text]

52. Woods, T.A., S.W. Graves, J.P. Nolan (2005) Protein surface concentration measurements using flow cytometry. Current Protocols in Cytometry 13.2.

53. Deshpande, A., Y.E. Valdez, J.P. Nolan (2005) SNP genotyping using single base extension and flow cytometry. Current Protocols in Cytometry 13.4.

54. Nolan J.P., A. Deshpande, F. Zhou (2005) Genetic Analysis Using Microsphere Arrays. In Flow Cytometry for Biotechnology (LA Sklar, ed) Oxford University Press.

55. Graves, S.W., J.P. Nolan, L.A. Sklar (2005) Molecular assembly analysis using flow cytometry. In Flow Cytometry for Biotechnology (LA Sklar, ed) Oxford University Press.

56. Yan X., W. Zhong, A. Tang, E.G. Schielke, W. Hang, J.P. Nolan (2005) Multiplexed flow cytometric immunoassay for influenza virus detection and differentiation. Analytical Chemistry 77: 7673-7678.  [Full Text]

57. Nolan, J.P., Mandy F. (2006) Multiplexed and microparticle-based analyses: Quantitative tools for the large-scale analysis of biological systems. Cytometry 69A:318-325.  [Full Text]

58. Saunders, M. J.,  H. Kim H, T. A. Woods, J.P. Nolan, L. A. Sklar, B. S. Edwards, S. W. Graves (2006) Microsphere-based protease assays and screening application for lethal factor and factor Xa. Cytometry 69A:342-352.  [Full Text]

59. Nolan, J.P., L. Yang, H.C. van der Heyde (2006) Reagents and instruments and for multiplexed analysis using microparticles. Current Protocols in Cytometry 13.8.

60. Goddard, G., J. C. Martin, M. Naivar, P. M. Goodwin, S. W. Graves, R.C. Habbersett, J.P. Nolan, J. H. Jett (2006) Spectral analysis flow cytometer: Modular inclusion of high resolution spectral analysis. Cytometry 69A: 842-851.  [Full Text]

61. Nolan, J.P. (2006) High throughput and high resolution molecular analysis using flow cytometry. Reviews in Fluorescence (in press).

62. van der Heyde, H.C., J.P. Nolan, V. Combes, I. Gramaglia, and G.E. Grau (2006) A unified hypothesis for the genesis of cerebral malaria: sequestration, inflammation, plus hemostasis leading to microcirculatory dysfunction. Trends in Parisitology Vol. 22, No. 11:503-508.  [Full Text]

63. Gramaglia, I., P. Sobelewski, D. Maeys, R. Contreras, J.P. Nolan, J. A. Frangos, M. Intaglietta, H. C. van der Heyde (2006) Low nitric oxide bioavailability contributes to the genesis of experimental cerebral malaria. Nature Medicine Vol. 12, No. 12:1417-1422.   [Full Text].

64. van der Heyde, H.C., W.P. Weidanz, J.M. Burns, I. Gramaglia, J.P. Nolan (2006) Analysis of antigen-specific antibodies and their isotypes in experimental malaria. Cytometry (in press).

REVIEW ARTICLES

1. Nolan, J.P., R.H. Hammerstedt (1997) Regulation of membrane stability and the acrosome reaction in mammalian sperm. FASEB Journal 11:670-682.

2. Nolan, J.P., L.A. Sklar (1998) The emergence of flow cytometry for sensitive, real-time measurements of molecular interactions.  Nature Biotechnology 16:633-638.

3. Nolan, J.P., S. Lauer, E.R. Prossnitz, L.A. Sklar (1999) Flow cytometry: a versatile tool for all stages of drug discovery. Drug Discovery Today 4:173-180.

4. Nolan J.P., Mandy F.F. (2001) Suspension array technology: New tools for gene and protein analysis. Cellular and Molecular Biology 47:1241-1256. 

5. Nolan, J.P., L.A. Sklar (2002) Suspension array technology: Evolution of the flat array paradigm. Trends in Biotechnology 20:9-12.

6. Sklar, L.A., B. Edwards, S.W. Graves, J.P. Nolan, E. Prossnitz (2002) Flow cytometric analysis of ligand-receptor interactions and molecular assemblies. Annual Review of Biophysics and Biomolecular Structure 31:97-119.

7. Cleland C.A., P.S. White, A. Deshpande, M. Wolinsky, J. Song, J.P. Nolan (2004) Rational design of nucleic acid signatures for the detection, identification, and characterization of microbial pathogens. Expert Reviews in Molecular Diagnostics 4: 89-101.

BOOK CHAPTERS

1. Nolan, J.P., J.D. Chambers, L.A. Sklar (1998) Cytometric approaches to the study of receptors. In, Cytometric Cellular Analysis, Vol. 1. Phagocyte Function: A Guide for Research and Clinical Evaluation (Eds. J.P. Robinson and G. Babcock), pp 19-46. John Wiley and Sons, New York.

2. Jett, J.H,  H. Cai, R.C. Habbersett, R.A. Keller, E.J. Larson, B.L. Marrone, J.P. Nolan, X. Song, B. Swanson, P.S. White (2001) Flow cytometry analysis techniques for high-throughput biodefense research.  In: Firepower in the Lab: Automation in the Fight Against Infectious Diseases and Bioterrorism.  (Layne SP, Beugelsdijk TJ, Patel CKN, eds).  Washington, DC: Joseph Henry Press, 193 - 201.

3. Nolan, J.P., P.S. White, H. Cai (2002) SNP scoring for drug discovery applications.  In, Integrated Drug Discovery Technologies (H.-Y. Mei and A. Czarnik, eds), pp 149-166, Marcel Dekker, New York.

4. Nolan J.P., A. Deshpande, F. Zhou (2004) Genetic Analysis Using Microsphere Arrays. In Flow Biotechnology (LA Sklar, ed) Academic Press, New York.  In press.

5. Graves, S.W., J.P. Nolan, L.A. Sklar (2004) Molecular assembly analysis using flow cytometry. In Flow Biotechnology (LA Sklar, ed) Academic Press, New York. In press.

PATENTS

1. Nolan JP, White PS, Cai H (2001) DNA polymorphism identity determination using flow cytometry. USPTO# 6,287,766

2. Nolan JP, Nolan RL, Ruscetti T, Lehnert BE (2002) Recombinant fluorescent protein microsphere calibration standard USPTO# 6,326,157.

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