Biochemistry & Molecular Biology
Flow Cytometry
Microcirculation
Microscopy & Cell Analysis

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Overview

Welcome to the La Jolla Bioengineering Core Facilities page.  Here you will find a partial list of our core lab areas and equipment. To register to use our facilities contact us at 858-456-7500.  If you have already registered, use the login link above to schedule a time to use our facilities.

Director: Gene Yeh, PhD
Instrumentation

• Protein Analysis:

  • Agilent Lab-on-a-chip

  • Biorad Fluor S

  • Biorad Protein Gel Electrophoresis

  • Akta Explorer 100 HPLC

  • Mass Spectrometer

• Nitirc Oxide Assessment

  • Sievers

  • Anaerobic Chamber with iNO probe

  • Saturated NO Solution

• Molecular Biology

  • Agilent Lab-on-a-chip (DNA and RNA)

  • Transfection Core

  • PCR

Director: John Nolan, PhD
Associate: Dakota Watson
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Instrumentation 

• Becton Dickenson FACSCalibur

• Becton Dickenson FACSort

• Union Biometrica COPAS Large Particle Sorter

• Luminex LX100

• Raman Flow Cytometer

• Small Particle Flow Cytometer

Director: Pedro Cabrales, PhD
Instrumentation

• Oximetery:

  • Vista Electronics Servonulling (implanted catheter): Blood pressure, heart rate, cardiac output

  • Xx (radiowave signal from catheter): Blood pressure, heart rate, movement, CO

• Macroscope: whole body detection of fluorescent signals

• Leica Intravital Microscope

• Whole Vessel Imaging:

  • Transonic Stress Imaging

  • Shear Stress Imaging

Director: Mirianas Chachisvilis, PhD
Associate: Pedro Cabrales, PhD
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Instrumentation

• Steady State Fluorescence and Absorption:

  • Fluorescence Spectrometer: Fluorolog 3 (Jobin-Yvon). 

  • Absorption spectrophotometer: Cary 3 (Varian, Inc.)

• Microscopy:

  • Inverted Fluorescence Microscope: Axiovert 200 M (Zeiss) 

  • Multiphoton Fluorescence Correlation Spectrometer

• Time Resolved Fluorescence Spectroscopy and Imaging:

  • Femtosecond Laser System for two-photon and time resolved microscopy (Spectra Physics, Inc.)

    • 11 W Pump Laser Millenia Xs with Tsunami Oscillator

    • OPAL Tunable Parametric Optical generator

    • Two Light Frequency Doublers

    • Pulse Selector: Model 3980

    • Multichannel Time Correlated Single Photon Counting Spectrograph

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