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Commercial
Instruments
Custom Instruments
Access, Scheduling & Training
Resources
Contact the Lab
Overview
The
LJBI Flow Cytometry Laboratory houses several multi-user commercial
instruments to support the research activities of the Institute and
its collaborators. These include analyzers and sorters for the
analysis of mammalian cells, bacteria, multicellular organisms, and
microsphere arrays. These instruments are available by appointment
to trained users. The Flow Cytometry Lab also houses
developmental custom instruments designed for specialized
applications.
Commercial
Instrumentation
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Becton
Dickenson FACSCalibur –
Measures four colors of fluorescence plus forward and
orthogonal light scatter. Automated sample loading and sorting
capabilities.
Excitation: 488, 635 nm.
Emission: 530/30, 585/30, 610+, 650+. |
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Becton
Dickenson FACSort – Measures three colors of
fluorescence plus forward and orthogonal light scatter.
Particle sorting capability.
Excitation: 488 nm.
Emission: 530/30,
585/42, 610+ |
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Union
Biometrica COPAS Large Particle Sorter
– Measures three colors of fluorescence plus orthogonal
scatter, extinction, and time of flight.
Excitation: 488, 514 nm.
Emission:
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Luminex
LX100 – Dedicated
instrument for analysis of microsphere arrays.
Measures three colors of fluorescence plus light
scatter.
Excitation: 532, 635 nm
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Developmental
Instrumentation
Raman
Flow Cytometer
This instrument development project to develop flow cytometers
capable of measuring Raman signals and spectra involves modified
commercial instruments that measure discrete Raman vibrations as
well as a prototype instrument for the analysis of complete optical
spectra.
Small
Particle Flow Cytometer
Based on the Los Alamos DNA Fragment Sizing Flow Cytometer, this
instrument is designed to measure small fluorescence and light
scatter signals from particles 1 um in diameter and below.
Access,
Training, and Scheduling
Commercial
instruments are available to approved users via the online
scheduling tool after appropriate training and orientation. Contact
the Lab by email to register as a new user.
Resources
Procedure
and Protocols
Current Protocols in Cytometry - http://www.mrw.interscience.wiley.com/cp/cpcy/cpcy_contents_fs.html
Cytometry
Links
Societies
Cytometry
Labs with Useful Web Sites
The
Scripps Research Institute http://facs.scripps.edu/facsindex.html
The Salk Institute - http://flowcyt.salk.edu/
Purdue University Flow Cytometry Lab http://www.cyto.purdue.edu/
Stanford University Shared FACS Facility - http://facs.stanford.edu/
Cardiff University - http://www.cardiff.ac.uk/medicine/haematology/cytonetuk/index.htm
University of Virginia - http://www.healthsystem.virginia.edu/internet/cytometry/
Contact
For
more information about the facility and its resources, email
us.
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