Steady State Fluorescence and Absorption

Fluorescence Spectrometer: Fluorolog 3 (Jobin-Yvon). Equipped with cooled PMT, four sample changer with temperature control, double excitation- and single- emission monochromators for best sensitivity.

Absorption spectrophotometer with sample temperature control and automated sample changer: Cary 3 (Varian, Inc.)

Microscopy

Inverted Fluorescence Microscope: Axiovert 200 M (Zeiss)
Equipped with confocal water immersion objective 40x C-Apochromat NA= 1.2

Developmental Instrumentation

Multiphoton Fluorescence Correlation Spectrometer
Complete with 6.25 ns time- and 0.4 mm spatial resolution and low-noise APD detectors (custom built). This spectrometer enables to record fluorescence auto- , cross- and dual-color correlation functions from live cells in order to characterize dynamics of molecular processes during signaling events in real time.

Time Resolved Fluorescence Spectroscopy and Imaging

Femtosecond Laser System for two-photon and time resolved microscopy: (Spectra Physics, Inc.) 

11 W Pump Laser Millenia Xs with Tsunami Oscillator:  Wavelength range 690 - 1100 nm, 80 MHz, < 100 fs laser source 

OPAL Tunable Parametric Optical Generator: 1180 - 2250 nm, 80 MHz, ~ 130 fs

Two Light Frequency Doublers: for upconverting fundamental laser light output to UV-Visible Wavelength range 340 nm - 700 nm for single photon excitation.

Pulse Selector: Model 3980
For reduction of laser pulse repetition rate below 80 MHz

Multichannel Time Correlated Single Photon Counting Spectrograph:
with 200 ps time resolution PML-16: (Becker & Hickl GmbH). This spectrometer enables detection of time resolved emission spectra from live cells and model membrane systems using single or two-photon excitation.